Pular para o conteúdo principal
Início

Dedicated to discovery

  • Sobre a ELGA
    • Sobre a ELGA
    • Carreiras
    • Eventos
  • Apoio
    • Planeamento laboratorial
  • Contacto
  • EN
  • DE
  • ES
  • FR
  • IT
  • EN-US
Início
  • Produtos
    • PURELAB
      • PURELAB® Dispenser
      • PURELAB® Flex
      • PURELAB® Quest
      • PURELAB® Pharma Compliance
      • PURELAB® Chorus 1 Complete
      • PURELAB® Chorus 1
      • PURELAB® Chorus 2
      • PURELAB® Chorus 3
      • PURELAB® Chorus 2 +
    • CENTRA
      • CENTRA® R60/120
      • CENTRA® R200
      • CENTRA® RDS
    • MEDICA
      • MEDICA® 150
      • MEDICA® 7/15
      • MEDICA® BIOX 2024
      • MEDICA® Pro-R & Pro-RE
      • MEDICA® R200
      • MEDICA® EDI 15/30
      • MEDICA® Pro-LPS
      • MEDICA® Pro EDI 60/120
      • MEDICA® BIOX
      • Hubgrade
    • BIOPURE
      • BIOPURE® 300/600
    • PURENERGY® 30
    • Gama completa dos produtos ELGA
  • Aplicações
    • Análise microbiológica
    • Bioquímica Clínica
    • Cromatografia gasosa
    • Cromatografia líquida
      • High Performance Liquid Chromatography
    • Culturas de célula
    • Eletroquímica
    • Espectrofotometria
    • Espectrometria de massa
    • Espectroscopia Atómica
    • Genética
    • High Performance Liquid Chromatography (HPLC)
    • Imunoquímica
    • Requisitos de água para laboratório em geral
  • Tecnologias
    • Carbono ativado
    • Eletrodeionização (EDI)
    • Filtração
    • Osmose Reversa
    • PureSure
    • Troca iónica
    • Ultravioleta
  • Impurezas na água
    • Compostos biológicos
    • Compostos inorgânicos
    • Gases dissolvidos
    • Microrganismos e bactérias
    • Partículas
  • Conhecimento
    • BROSCHÜREN
    • Blogue
    • Estudos de caso
    • Água ultrapura
    • Guias e Livros brancos
  • Where to buy
  • Contacto
Home
  • Contacto
  • Produtos
    • PURELAB
      • PURELAB® Dispenser
      • PURELAB® Flex
      • PURELAB® Quest
      • PURELAB® Pharma Compliance
      • PURELAB® Chorus 1 Complete
      • PURELAB® Chorus 1
      • PURELAB® Chorus 2
      • PURELAB® Chorus 3
      • PURELAB® Chorus 2 +
    • CENTRA
      • CENTRA® R60/120
      • CENTRA® R200
      • CENTRA® RDS
    • MEDICA
      • MEDICA® 150
      • MEDICA® 7/15
      • MEDICA® BIOX 2024
      • MEDICA® Pro-R & Pro-RE
      • MEDICA® R200
      • MEDICA® EDI 15/30
      • MEDICA® Pro-LPS
      • MEDICA® Pro EDI 60/120
      • MEDICA® BIOX
      • Hubgrade
    • BIOPURE
      • BIOPURE® 300/600
    • PURENERGY® 30
    • Gama completa dos produtos ELGA
  • Aplicações
    • Análise microbiológica
    • Bioquímica Clínica
    • Cromatografia gasosa
    • Cromatografia líquida
      • High Performance Liquid Chromatography
    • Culturas de célula
    • Eletroquímica
    • Espectrofotometria
    • Espectrometria de massa
    • Espectroscopia Atómica
    • Genética
    • High Performance Liquid Chromatography (HPLC)
    • Imunoquímica
    • Requisitos de água para laboratório em geral
  • Tecnologias
    • Carbono ativado
    • Eletrodeionização (EDI)
    • Filtração
    • Osmose Reversa
    • PureSure
    • Troca iónica
    • Ultravioleta
  • Impurezas na água
    • Compostos biológicos
    • Compostos inorgânicos
    • Gases dissolvidos
    • Microrganismos e bactérias
    • Partículas
  • Conhecimento
    • BROSCHÜREN
    • Blogue
    • Estudos de caso
    • Água ultrapura
    • Guias e Livros brancos
  • Where to buy
  • Contacto
  • Sobre a ELGA
    • Sobre a ELGA
    • Carreiras
    • Eventos
  • Apoio
    • Planeamento laboratorial
  • Contacto
  • EN
  • DE
  • ES
  • FR
  • IT
  • EN-US
  • Política de privacidade
  • Termos e condições
  • Conformidade legal global
  • Patentes
  • Trademarks
  • Impressum
  • ELGA PURELAB Helps Standardize Antibiotic Susceptibility Testing
Clinical & Pharma
Science of the Future

ELGA PURELAB Helps Standardize Antibiotic Susceptibility Testing

29 fev 2020
- by Dr Alison Halliday

Female scientist analyzing growing bacterial cultures in petri dish in laboratory, science and microbiology background

Using Ultrapure Water in Antibiotic Susceptibility Testing

A new study makes recommendations for combining two different methods to test bacterial susceptibility to antibiotics, which could help improve reproducibility between laboratories and provide more clinically relevant information for treating infections.

Every day, thousands of experimental and diagnostic laboratories around the world are carrying out antimicrobial susceptibility testing (AST) to search for an effective antibiotic to use against a bacterial strain. But results can vary widely between laboratories, and there is a need to create standardized protocols to improve reproducibility and reliability of results.

One of the main techniques used by laboratories is measuring the minimum inhibitory concentration (MIC). This gauges the lowest antibiotic concentration that inhibits the visible growth of a microorganism after incubation for 16-20 hours.  But this test has limitations - it doesn’t reveal whether the drug kills the bacteria, or just halts its growth. And crucially, it’s not good at predicting the antibiotic’s actual effectiveness within the body.

A Killer Concentration

Another measure is to define an antibiotic’s minimal bactericidal concentration (MBC), which defines the lowest concentration that’s needed to kill the bacteria. This is especially important for patients with inflammatory diseases or who are immunocompromised - as their immune system may not be working well enough to fully eliminate the pathogens should an antibiotic only inhibit its growth.

Determining the MBC involves a test to work out the lowest concentration of an antibiotic that reduces the number of bacterial colonies on subculture by 99.9%. But this test attracts criticism, due to its poor reproducibility between laboratories. Involving broth dilution and re-growth of bacteria in antibiotic-free media, potential variables include the size of the inoculum, sample mixing and growth phase of the bacteria.

Combining MBC and MIC Approaches

Assessing both the MBC and MIC may be a more useful strategy for determining the clinical relevance of an antibiotic. A new study, published in the Journal of Microbiological Methods, carried out an objective, detailed comparison of a range of different methodologies for determining the MIC and MBC (1). 

The researchers tested two bacterial strains, P.aeruginosa PA01 and E.coli ATCC 25922 against four antibiotics: gentamicin, tobramycin, ciprofloxacin, and colistin – as well as S.aureus, which they additionally tested with vancomycin. They used ultrapure water purified using PURELAB Flex apparatus from ELGA LabWater for the preparation and analysis of all samples.

The team worked out the MIC using broth microdilution, a calorimetric method and a microscopy-based screening system (MBSS). For determining the MBC, they carried out flurophore staining, comparing this with the broth regrowth method.

New Recommendations to Assess Antibiotics

After evaluating their results, the researchers recommend different combinations of methods to determine the MIC and MBC, depending on whether time, cost or sensitivity is the main priority. They also emphasize the importance of uniform protocols across different laboratories, including a suggested standardization of the timepoint for reading MIC results of 20 hours.

This important new study proposes new recommendations for the best methods to assess which antibiotics are effective against a bacterial strain. These will help improve reproducibility across laboratories, gain more clinically relevant data, and enable a better understanding of the action of antibiotic drugs.

Why Choose ELGA LabWater?

We are the LabWater Specialists since 1937 we have been working with scientists to guarantee pure and ultrapure water for their experiments and lab work. Laboratories around the world trust our laboratory water purification systems to help their researchers to achieve accurate, reliable results.


References

(1)  Klodzinska et al. Combining diagnostic methods for antimicrobial susceptibility testing – a comparative approach. Journal of Microbiological Methods 144 (2018), p177-185.
 

Dr Alison Halliday
After completing an undergraduate degree in Biochemistry & Genetics at Sheffield University, Alison was awarded a PhD in Human Molecular Genetics at the University of Newcastle. She carried out five years as a Senior Postdoctoral Research Fellow at UCL, investigating the genes involved in childhood obesity syndrome. Moving into science communications, she spent ten years at Cancer Research UK engaging the public about the charity’s work. She now specialises in writing about research across the life sciences, medicine and health.

  • Sales Enquiry
  • Obter uma cotação
  • Technical Support
  • Encontre um parceiro aprovado

Sales Enquiry

At ELGA LabWater, we have exciting offers and news about our products and services that we hope you’d like to hear about. We will use your information to predict what you might be interested in. We will treat your data with respect and you can find the details in our Privacy Policy.

ELGA LabWater works with a network of Approved Partners. In order to answer your questions or enquiries, we may pass your contact details to an Approved Partner, who may contact you directly. 

I'd like to receive updates from ELGA LabWater & ELGA Approved Partners based on my details.

Obter uma cotação

At ELGA LabWater, we have exciting offers and news about our products and services that we hope you’d like to hear about. We will use your information to predict what you might be interested in. We will treat your data with respect and you can find the details in our Privacy Policy.

ELGA LabWater works with a network of Approved Partners. In order to answer your questions or enquiries, we may pass your contact details to an Approved Partner, who may contact you directly. 

I'd like to receive updates from ELGA LabWater & ELGA Approved Partners based on my details.

Technical Support

At ELGA LabWater, we have exciting offers and news about our products and services that we hope you’d like to hear about. We will use your information to predict what you might be interested in. We will treat your data with respect and you can find the details in our Privacy Policy.

ELGA LabWater works with a network of Approved Partners. In order to answer your questions or enquiries, we may pass your contact details to an Approved Partner, who may contact you directly. 

Call us

Can't find what you are looking for?

Support Number
+44 (0)20 3567 7300
United Kingdom Sales
+44 (0)1628 879 704
United States of America Sales
+1 877-315-3542
France Sales
+33 1 40 83 65 00
China Sales
+86 400-616-8882

 

Sede da ELGA LabWater

Lane End Business Park
Lane End, High Wycombe
HP14 3BY
Reino Unido
T: +44 (0) 203 567 7300
F: +44 (0) 203 567 7205

Estudos de caso

  • Abbott Diagnostics
  • DASA Medical Diagnostics
  • NeoDIN Medical Institute
  • North Staffordshire NHS Trust
  • Olsberg Vocational College

Recursos

  • Saber mais sobre água ultrapura
  • Guias e Livros Brancos
  • Tecnologias de purificação
  • Aplicações
  • Impurezas na água
  • Latest Blog
  • Water Purity - Different Types of Pure Water
  • What is Clinical Laboratory Reagent Water (CLSI)?
  • What is Total Organic Carbon (TOC)?

© VWS (UK) Ltd. negociando como ELGA LabWater.2025-Todos os direitos reservados.
ELGA é a marca global de água para laboratórios da Veolia.

  • Política de privacidade
  • Termos e condições
  • Conformidade legal global
  • Patentes
  • Trademarks
  • Impressum
  • Idioma
    • Deutsch
    • English
    • Español
    • Français
    • Italiano
    • Português
    • 日本語
    • 中文
  • Outros sítios Web da Veolia
    • Veolia
    • Veolia Foundation
    • Veolia Water Technologies
Elga Veolia
TOP

© 2017 ELGA Veolia