Pular para o conteúdo principal
  • Parceiros aprovados
  • Sobre a ELGA
    • Sobre a ELGA
    • Carreiras
    • Eventos
  • Apoio
    • Planeamento laboratorial
    • Registar um produto
    • Register Your Product (USA & Canada Only)
  • Contacto
  • English
  • Deutsch
  • Español
  • Français
  • Italiano
  • 日本語
  • 中文
Início ELGA LabWater
  • Produtos
    • PURELAB
    • CENTRA
    • MEDICA
    • BIOPURE
    • Gama completa dos produtos ELGA
  • Aplicações
    • Análise microbiológica
    • Bioquímica Clínica
    • Cromatografia gasosa
    • Cromatografia líquida
    • Culturas de célula
    • Eletroquímica
    • Espectrofotometria
    • Espectrometria de massa
    • Espectroscopia Atómica
    • Genética
    • Imunoquímica
    • Requisitos de água para laboratório em geral
  • Tecnologias
    • Carbono ativado
    • Eletrodeionização (EDI)
    • Filtração
    • Osmose Reversa
    • PureSure
    • Troca iónica
    • Ultravioleta
  • Impurezas na água
    • Compostos biológicos
    • Compostos inorgânicos
    • Gases dissolvidos
    • Microrganismos e bactérias
    • Partículas
  • Knowledge
    • Blogue
    • Estudos de caso
    • Água ultrapura
    • Guias e Livros brancos
  • Produtos
    • PURELAB
      • PURELAB® flex 1 e 2
      • PURELAB® flex 3 & 4
    • CENTRA
      • CENTRA® 60/120
      • CENTRA® RDS
      • CENTRA® R200
    • MEDICA
      • MEDICA® 7/15
      • MEDICA® EDI 15/30
      • MEDICA® Pro EDI 60/120
      • MEDICA® Pro-LPS
      • MEDICA® Pro-R e Pro-RE
      • MEDICA® R200
    • BIOPURE
      • BIOPURE 7/15
      • BIOPURE 60/120
      • BIOPURE 200/300/600
    • Gama completa dos produtos ELGA
      • PURELAB® Classic
  • Aplicações
    • Análise microbiológica
    • Bioquímica Clínica
    • Cromatografia gasosa
    • Cromatografia líquida
    • Culturas de célula
    • Eletroquímica
    • Espectrofotometria
    • Espectrometria de massa
    • Espectroscopia Atómica
    • Genética
    • Imunoquímica
    • Requisitos de água para laboratório em geral
  • Tecnologias
    • Carbono ativado
    • Eletrodeionização (EDI)
    • Filtração
    • Osmose Reversa
    • PureSure
    • Troca iónica
    • Ultravioleta
  • Impurezas na água
    • Compostos biológicos
    • Compostos inorgânicos
    • Gases dissolvidos
    • Microrganismos e bactérias
    • Partículas
  • Knowledge
    • Blogue
      • Analytical Chemistry
      • Clinical & Pharma
      • Cool Science
      • Environment and sustainability
      • Life in the lab
      • Purelab product design
      • Science of the future
      • Water Purity
      • Water in the lab
    • Estudos de caso
      • A Abbott Diagnostics escolhe os sistemas ELGA MEDICA no Sul da Ásia
      • Beam Me Up, Scotty: PURELAB® Option Q Delivers Essential Ultra Pure Water
      • Clean Water for a Clean Future
      • Critital Tests Benefit from PURELAB® Option Reliable Pure Water
      • DASA: a maior empresa de diagnósticos médicos do Brasil
      • A ELGA ajuda os laboratórios de imunosserologia a maximizar o tempo de atividade
      • Fondazione Telethon Continues to Choose ELGA Labwater as a Trusted Partner
      • Importância da água ultrapura Tipo 1+ para o desenvolvimento de medicamentos genéricos
      • MEDICA® Pro selecionado para analisadores Siemens ADVIA® no City General Hospital
      • Optimale Wasserqualität für mikrobiologische Forschung und Lehre
      • PURELAB® Option R Guarantees Pure Water for Leading Microfluidics Technology
      • PURELAB® Pulse Delivers Reliable Water Quality and Quantity for a Wide Range of Applications
      • PURELAB® flex: um sistema de formação ideal para o método de investigação atual
      • Powering Cutting-Edge Gene Research
      • Sichere Reinstwasserversorgung für präzise Produkttests
      • Zentrale Reinstwasser-Aufbereitung für Analyser
      • ELGA LabWater and Beckman Coulter Join Forces
      • Applied New Technologies Department Improves ICP, IC & HPLC Sample Turnaround Times with PURELAB®
      • Desvendar os segredos da Antártida com a ajuda de água ultrapura
      • Advancing Genetic Technologies
      • Cross Infection Control: Pure and Simple
      • Researching effective new ways to prevent cardiovascular disease at the University of Columbia
    • Água ultrapura
    • Guias e Livros brancos
      • Pureza da água de HPLC
      • HPLC no setor farmacêutico
      • Redução de riscos clínicos
      • Sustentabilidade
  • English
  • Deutsch
  • Español
  • Français
  • Italiano
  • 日本語
  • 中文
  • Política de privacidade
  • Termos e condições
  • Conformidade legal global
  • Patentes
  • Impressum
  • Início
  • Node
  • Lipodendriplexes: A Promising Alternative For Gene Therapy
Clinical & Pharma
Science of the Future

Lipodendriplexes: A Promising Alternative For Gene Therapy

5 jan 2020
- by Dr Alison Halliday

DNA

Gene therapies hold tremendous promise for patients with many diseases – from genetic disorders to cancers. Non-viral vectors may offer a safe, efficient and non-toxic alternative to using viruses to deliver genes into target cells.

Scientists are developing new ways to modify a patient’s cells genetically to help fight a disease. Viruses are by far the most efficient biological vectors to deliver a gene into target cells. Despite years of research, very few gene therapies are approved for clinical use, largely due to safety concerns around the use of viral vectors. But there is currently a lack of efficient non-viral vector delivery systems that could be used as safer alternatives. Researchers are actively exploring a wide range of delivery strategies and compounds to develop new systems with the ideal properties for gene transfer.

Combining Forces

Free DNA is unable to get inside cells and is normally degraded in the blood circulation before it can reach and enter target cells. Synthetic vectors can offer protection from destruction by forming stable complexes with nucleic acids. These can then efficiently get inside cells, releasing their cargo to achieve the desired therapeutic effect.

Polyamidoamine (PAMAM) dendrimer, a biodegradable polymer that has a tendency to complex with nucleic acids (forming dendriplexes), is of the most extensively investigated synthetic vectors for gene therapy applications. Liposomal formulations are also well-known approach for the delivery of drugs and genetic materials into cells.

However, dendriplexes and liposomes both have properties that can affect their safety and efficiency. Creating a lipid coated polymer system that exploits the benefits of both liposomes and PAMAM dendrimers (lipodendriplexes) could prove a more effective strategy. 

More Efficient, Safer Gene Transfer

In a new study, published in the European Journal of Pharmaceutics and Biopharmaceutics, a team of scientists set out to establish an optimised lipodendriplex system.1 The researchers incorporated PAMAM dendriplexes in a broad range of liposomal formulations, characterising their physicochemical properties including size, shape and zeta potential. This was done using dynamic light scattering, laser Doppler anemometry and atomic force microscopy. They then carried out a series of in vitro experiments to identify the best formulation that showed the optimal delivery efficiency combined with the lowest cell toxicity.

The team next demonstrated that their optimised liposome encapsulated dendriplex system can further reduce unwanted cell toxicity and enhanced nucleic acid uptake compared with the parent dendriplex system. They also carried out initial safety assays, with the results indicating it has a good biocompatibility profile. To avoid introducing contaminants that may affect their results, the team used analytical grade reagents and purified water from an ELGA PURELAB® flex 2 water purification system for all experiments.

A Potential Alternative to Viral Vectors 

This study suggests that lipodendriplexes could offer an important alternative for gene therapy applications to viral vectors – with the potential for efficient gene transfer with a better safety profile. The next steps will be to carry out in vivo studies to further explore the potential of this platform using specific gene targeted systems against different cancers and genetic disorders.

Why choose ELGA LabWater?

ELGA’s expert engineers, chemists and scientists are at the forefront of technological innovation. We continue to introduce game-changing features to the laboratory ultrapure water market.

Reference:

  1. Tariq, I. et al. Lipodendriplexes: A promising nanocarrier for enhanced gene delivery with minimal toxicity. Eur J Pharm Biopharm 2019; 135:72-82.


Dr Alison Halliday
After completing an undergraduate degree in Biochemistry & Genetics at Sheffield University, Alison was awarded a PhD in Human Molecular Genetics at the University of Newcastle. She carried out five years as a Senior Postdoctoral Research Fellow at UCL, investigating the genes involved in childhood obesity syndrome. Moving into science communications, she spent ten years at Cancer Research UK engaging the public about the charity’s work. She now specialises in writing about research across the life sciences, medicine and health.

 

  • Enquiry
  • Obter uma cotação
  • Agende uma demonstração
  • Encontre um parceiro aprovado

Enquiry

Please check this to confirm that you have read our Terms of Service and Privacy Policy.

Obter uma cotação

Please check this to confirm that you have read our Terms of Service and Privacy Policy.

Agende uma demonstração

Please check this to confirm that you have read our Terms of Service and Privacy Policy.

Call us

Can't find what you are looking for?

Support Number
+44 (0)20 3567 7300
United Kingdom Sales
+44 (0)1628 879 704
United States of America Sales
+1 877-315-3542
France Sales
+33 1 40 83 65 00
China Sales
+86 400-616-8882

 

Sede da ELGA LabWater

Lane End Business Park
Lane End, High Wycombe
HP14 3BY
Reino Unido
T: +44 (0) 203 567 7300
F: +44 (0) 203 567 7205

Os especialistas em água ultrapura

  • Apoio e serviços
  • Eventos

Estudos de caso

  • Abbott Diagnostics
  • DASA Medical Diagnostics
  • NeoDIN Medical Institute
  • North Staffordshire NHS Trust
  • Olsberg Vocational College

Recursos

  • Saber mais sobre água ultrapura
  • Guias e Livros Brancos
  • Tecnologias de purificação
  • Aplicações
  • Impurezas na água

Blogs

  • Latest Blog
  • Water Purity - Different Types of Pure Water
  • What is Clinical Laboratory Reagent Water (CLSI)?
  • What is Total Organic Carbon (TOC)?
  • Idioma
    • Deutsch
    • English
    • Español
    • Français
    • Italiano
    • Português
    • 日本語
    • 中文
  • Outros sítios Web da Veolia
    • Veolia
    • Veolia Fondation
    • Veolia Water Technologies

© VWS (UK) Ltd. negociando como ELGA LabWater.2021-Todos os direitos reservados.
ELGA é a marca global de água para laboratórios da Veolia.

  • Política de privacidade
  • Termos e condições
  • Conformidade legal global
  • Patentes
  • Impressum
Elga Veolia
TOP

© 2017 ELGA Veolia